HSP70基因在人胃癌、食道癌及宫颈癌中高表达和癌变关系的研究

doi:10.3969/j.issn.1004-616x.2005.04.005

Carcinogenesis, Teratogenesis & Mutagenesis ›› 2005, Vol. 17 ›› Issue (4): 211-212.doi: 10.3969/j.issn.1004-616x.2005.04.005

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High Level Expression of HSP70 Gene in Tissues of Gastric, Esophageal and

CHEN Lan-ying, ZHAO Zhen, WANG Xiao-tao

Pingdingshan Technological Institute，Pingdingshan

Received:2004-11-30 Revised:2005-02-28 Online:2005-07-30 Published:2005-07-30
Contact: CHEN Jian-nan

Abstract

Abstract: BACKGROUND ＆ AIM: To study the expression of HSP70 gene from tissues of Gastric carcinoma,Esophagus carcinoma and Cervical carcinoma. MATRAIAL AND METHODS: Tissue samples of cancers(gastric carcinoma, esophagus cancer, cervical carcinoma)were obtained from operating room of hospital.RNA was extracted according to the cyanic acid guanidine law of different sulphur separately,RNA separation carried out with 1 ％ agarose electrophoresis.RNA was transfered from the straining gel to (Northern blot ) the membrane , and then using HSP70 gene probe P17 was applied to the RNA from gastric carcinoma,esophagus carcinoma and cervical carcinoma. RESULTS: The results indicates that HSP70 gene plays an important role in the development of cancer. It also provided valuable clues for the understanding of mechanism of cancer genesis and gene treatment for cancer.

Key words: expressing of HSP70 gene, Northern blotting, gastric carcinoma, esophagus cancer, cervical carcinoma

CHEN Lan-ying, ZHAO Zhen, WANG Xiao-tao. High Level Expression of HSP70 Gene in Tissues of Gastric, Esophageal and[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2005, 17(4): 211-212.

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High Level Expression of HSP70 Gene in Tissues of Gastric, Esophageal and

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